Monitoring lipophagy in kidney epithelial cells in response to shear stress.
Methods Cell Biol
; 164: 11-25, 2021.
Article
en En
| MEDLINE
| ID: mdl-34225909
ABSTRACT
Mechanical stress has been shown to induce the degradation of lipid droplets in kidney epithelial cells. Here, we illustrate the technical equipment and devices that are currently used in our laboratory to apply shear stress on cells. We provide a detailed protocol to monitor lipophagy in response to shear stress. The aim of this review is to guide and help people understand the challenges in studying acidic lipolysis in cells subjected to fluid flow.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Autofagia
/
Metabolismo de los Lípidos
Límite:
Humans
Idioma:
En
Revista:
Methods Cell Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Francia