In Vitro Reconstitution of the Pantothenic Acid Degradation Pathway in Ochrobactrum anthropi.
ACS Chem Biol
; 16(8): 1350-1353, 2021 08 20.
Article
en En
| MEDLINE
| ID: mdl-34313416
ABSTRACT
Pantothenic acid is an essential metabolite found throughout all branches of life. Although the enzymes responsible for pantothenic biosynthesis have been characterized, those leading to its biodegradation remain poorly understood. In the study described herein, we showed that use of a "genomic enzymology" strategy enabled identification of four biodegradation pathway genes, which were then confirmed by using kinetic analysis of the purified recombinant enzymes encoded in Ochrobactrum anthropi. The reconstituted pathway converts pantothenic acid to ß-alanine and (R)-pantoate, and then (R)-pantoate to aldopentoate, which is transformed to (R)-3,3-dimethylmalate and hence to α-ketoisovalerate. The pathway genes are common to Proteobacterial genomes in which they are not colocated.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ácido Pantoténico
/
Ochrobactrum anthropi
Idioma:
En
Revista:
ACS Chem Biol
Año:
2021
Tipo del documento:
Article
País de afiliación:
China