Echinacoside protects adenine-induced uremic rats from sciatic nerve damage by up-regulating α-Klotho.
J Musculoskelet Neuronal Interact
; 21(3): 413-421, 2021 09 01.
Article
en En
| MEDLINE
| ID: mdl-34465681
ABSTRACT
OBJECTIVES:
To investigate the therapeutic effect of Echinacoside on uremia-induced sciatic nerve injury and explore the specific molecular mechanism and role of α-Klotho.METHODS:
SD rats were given continuous gavage of adenine to prepare a uremia-induced sciatic nerve injury model. The model was given either Echinacoside or α-Klotho by gavage. Histopathological changes of kidney and sciatic nerve were detected by H&E staining. The changes of creatinine, urea nitrogen, and urine protein were detected by biochemical detection. The changes of IL-1ß and IL-18 were detected by ELISA. Nerve activity-related indicators were detected by biochemical detection. Changes in related mRNA and protein expression were detected by qPCR and western blot.RESULTS:
Creatinine, urea nitrogen, urine protein, and malondialdehyde (MDA) in the model group were significantly increased and inhibited by Echinacoside and α-Klotho treatment with Echinacoside dose-dependence. Meanwhile, the activities of ATP concentration, potassium adenosine triphosphate (Na+, K+ ATPase), succinate dehydrogenase (SDH), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) showed opposite trends.CONCLUSIONS:
Echinacoside can significantly relieve uremia-induced sciatic nerve injury in rats. Its specific molecular mechanism is related to the inhibition of the classical cellular pyroptosis pathway, which is likely achieved by promoting α-Klotho expression.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nervio Ciático
/
Adenina
Límite:
Animals
Idioma:
En
Revista:
J Musculoskelet Neuronal Interact
Asunto de la revista:
FISIOLOGIA
/
NEUROLOGIA
/
ORTOPEDIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
China