Inside the Protein Corona: From Binding Parameters to Unstained Hard and Soft Coronas Visualization.
Nano Lett
; 21(19): 8250-8257, 2021 10 13.
Article
en En
| MEDLINE
| ID: mdl-34554750
ABSTRACT
Proteins spontaneously adsorb on nanoparticle surfaces when injected into the bloodstream. It drastically modifies the nanoparticle's fate and how they interact with organs and cells. Although this protein layer (protein corona) has been widely studied, the robustness of the most employed characterization methods and the visualization of its unstained fractions remain open questions. Here, synchrotron-based small-angle X-ray scattering was used to follow the corona formation and estimate binding parameters. At the same time, transmission electron microscopy under cryogenic conditions associated with cross-correlation image processing and energy-filtered transmission electron microscopy allowed to determine protein corona morphology and thickness together with the visualization of its unstained hard and soft fractions. The above-presented strategy shows tremendous potential for deciphering fundamental protein corona aspects and can contribute to rational medical nanoparticle engineering.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Nanopartículas
/
Corona de Proteínas
Idioma:
En
Revista:
Nano Lett
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil