5-Aminolevulinic acid level and dye-decolorizing peroxidase expression regulate heme synthesis in Escherichia coli.
Biotechnol Lett
; 44(2): 271-277, 2022 Feb.
Article
en En
| MEDLINE
| ID: mdl-34826004
ABSTRACT
OBJECTIVES:
To investigate the level of 5-aminolevulinic acid (5-ALA), a key precursor of heme, and expression of heme-peroxidase on the regulation of heme synthesis in E. coli.METHODS:
A transporter gene (eamA) was knocked out, and glutamyl-tRNA reductase gene (hemA) for 5-ALA synthesis and a dye-decolorizing peroxidase gene (DyP) were overexpressed.RESULTS:
Knockout of eamA caused decrease of 5-ALA secretion, indicating EamA participates in 5-ALA transportation. Overexpression of hemA elevated intracellular 5-ALA and heme levels. However, overexpression of hemA in eamA knockout mutant led to decrease of intracellular heme content and down-regulation of the transcription of heme synthetic gene hemL by ~ 5.2-fold. When overexpressing both hemA and DyP in the mutant, hemL was up-regulated suggesting the binding of heme to DyP released the feedback repression of hemL.CONCLUSION:
HemL expression is heme-mediated and the approach of intracellular immobilization of free heme by overexpression of heme-peroxidase benefits the understanding and application of heme regulation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Escherichia coli
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Escherichia coli
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Ácido Aminolevulínico
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Hemo
Idioma:
En
Revista:
Biotechnol Lett
Año:
2022
Tipo del documento:
Article
País de afiliación:
China