A Hyperthermoactive-Cas9 Editing Tool Reveals the Role of a Unique Arsenite Methyltransferase in the Arsenic Resistance System of Thermus thermophilus HB27.

Gallo, Giovanni; Mougiakos, Ioannis; Bianco, Mauricio; Carbonaro, Miriam; Carpentieri, Andrea; Illiano, Anna; Pucci, Pietro; Bartolucci, Simonetta; van der Oost, John; Fiorentino, Gabriella

Gallo, Giovanni; Mougiakos, Ioannis; Bianco, Mauricio; Carbonaro, Miriam; Carpentieri, Andrea; Illiano, Anna; Pucci, Pietro; Bartolucci, Simonetta; van der Oost, John; Fiorentino, Gabriella.

Afiliación

Gallo G; Department of Biology, University of Naples Federico IIgrid.4691.a, Naples, Italy.
Mougiakos I; Consiglio Nazionale delle Ricerche CNR, Institute of Polymers, Composites and Biomaterials (IPCB), Pozzuoli, Naples, Italy.
Bianco M; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
Carbonaro M; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), Würzburg, Germany.
Carpentieri A; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
Illiano A; Department of Biology, University of Naples Federico IIgrid.4691.a, Naples, Italy.
Pucci P; Department of Chemical Sciences, University of Naples Federico IIgrid.4691.a, Naples, Italy.
Bartolucci S; CEINGE-Biotecnologie avanzate, Naples, Italy.
van der Oost J; CEINGE-Biotecnologie avanzate, Naples, Italy.
Fiorentino G; Department of Biology, University of Naples Federico IIgrid.4691.a, Naples, Italy.

mBio ; 12(6): e0281321, 2021 12 21.

Article en En | MEDLINE | ID: mdl-34872358

Asunto(s)

Arsénico/metabolismo; Proteínas Bacterianas/química; Proteínas Bacterianas/genética; Metiltransferasas/química; Metiltransferasas/genética; Thermus thermophilus/enzimología; Secuencia de Aminoácidos; Arsénico/química; Proteínas Bacterianas/metabolismo; Sitios de Unión; Sistemas CRISPR-Cas; Estabilidad de Enzimas; Edición Génica; Metiltransferasas/metabolismo; Alineación de Secuencia; Thermus thermophilus/química; Thermus thermophilus/genética

Palabras clave

CRISPR-Cas9 genome editing; Thermus thermophilus; arsenic resistance; bioreporter; extremophiles; genetic tool; thermoresistance

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Arsénico / Proteínas Bacterianas / Thermus thermophilus / Metiltransferasas Idioma: En Revista: MBio Año: 2021 Tipo del documento: Article País de afiliación: Italia

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