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LFA1 and ICAM1 are critical for fusion and spread of murine leukemia virus in vivo.
Engels, Rebecca; Falk, Lisa; Albanese, Manuel; Keppler, Oliver T; Sewald, Xaver.
Afiliación
  • Engels R; LMU München, Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, Munich, Germany.
  • Falk L; LMU München, Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, Munich, Germany.
  • Albanese M; LMU München, Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, Munich, Germany.
  • Keppler OT; LMU München, Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, Munich, Germany.
  • Sewald X; LMU München, Max von Pettenkofer Institute & Gene Center, Virology, National Reference Center for Retroviruses, Munich, Germany. Electronic address: sewald@mvp.lmu.de.
Cell Rep ; 38(3): 110279, 2022 01 18.
Article en En | MEDLINE | ID: mdl-35045303
Murine leukemia virus (MLV)-presenting cells form stable intercellular contacts with target cells during infection of lymphoid tissue, indicating a role of cell-cell contacts in retrovirus dissemination. Whether host cell adhesion proteins are required for retrovirus spread in vivo remains unknown. Here, we demonstrate that the lymphocyte-function-associated-antigen-1 (LFA1) and its ligand intercellular-adhesion-molecule-1 (ICAM1) are important for cell-contact-dependent transmission of MLV between leukocytes. Infection experiments in LFA1- and ICAM1-deficient mice demonstrate a defect in MLV spread within lymph nodes. Co-culture of primary leukocytes reveals a specific requirement for ICAM1 on donor cells and LFA1 on target cells for cell-contact-dependent spread through trans- and cis-infection. Importantly, adoptive transfer experiments combined with a newly established MLV-fusion assay confirm that the directed LFA1-ICAM1 interaction is important for retrovirus fusion and transmission in vivo. Taken together, our data provide insights on how retroviruses exploit host proteins and the biology of cell-cell interactions for dissemination.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Leucemia Experimental / Antígeno-1 Asociado a Función de Linfocito / Virus de la Leucemia Murina / Infecciones por Retroviridae / Molécula 1 de Adhesión Intercelular Límite: Animals Idioma: En Revista: Cell Rep Año: 2022 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Leucemia Experimental / Antígeno-1 Asociado a Función de Linfocito / Virus de la Leucemia Murina / Infecciones por Retroviridae / Molécula 1 de Adhesión Intercelular Límite: Animals Idioma: En Revista: Cell Rep Año: 2022 Tipo del documento: Article País de afiliación: Alemania