Generation of a <i>NES</i>-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing.

Nouri, Parivash; Zimmer, Anja; Brüggemann, Stefanie; Friedrich, Robin; Kühn, Ralf; Prakash, Nilima

Generation of a NES-mScarlet Red Fluorescent Reporter Human iPSC Line for Live Cell Imaging and Flow Cytometric Analysis and Sorting Using CRISPR-Cas9-Mediated Gene Editing.

Nouri, Parivash; Zimmer, Anja; Brüggemann, Stefanie; Friedrich, Robin; Kühn, Ralf; Prakash, Nilima.

Afiliación

Nouri P; Laboratory of Applied Genetics and Stem Cell Biology, Department Hamm 2, Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany.
Zimmer A; Genome Engineering & Disease Models, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany.
Brüggemann S; Laboratory of Applied Genetics and Stem Cell Biology, Department Hamm 2, Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany.
Friedrich R; Laboratory of Applied Genetics and Stem Cell Biology, Department Hamm 2, Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany.
Kühn R; Genome Engineering & Disease Models, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany.
Prakash N; Laboratory of Applied Genetics and Stem Cell Biology, Department Hamm 2, Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany.

Cells ; 11(2)2022 01 13.

Article en En | MEDLINE | ID: mdl-35053384

Asunto(s)

Sistemas CRISPR-Cas/genética; Citometría de Flujo; Edición Génica; Genes Reporteros; Células Madre Pluripotentes Inducidas/metabolismo; Proteínas Luminiscentes/metabolismo; Nestina/metabolismo; Calcio/metabolismo; Diferenciación Celular/genética; Línea Celular; Neuronas Dopaminérgicas/metabolismo; Regulación de la Expresión Génica; Humanos; Mesencéfalo/metabolismo; Células-Madre Neurales/metabolismo; Proteína Fluorescente Roja

Palabras clave

CRISPR-Cas9; NESTIN; iPSC; midbrain dopamine neurons; neural stem cells; reporter

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Genes Reporteros / Células Madre Pluripotentes Inducidas / Nestina / Sistemas CRISPR-Cas / Citometría de Flujo / Edición Génica / Proteínas Luminiscentes Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cells Año: 2022 Tipo del documento: Article País de afiliación: Alemania

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