Novel long non-coding RNA lncAMPC downregulates PTEN via miR-214 to promote nasopharyngeal carcinoma progression.
Mol Cell Biochem
; 477(3): 805-814, 2022 Mar.
Article
en En
| MEDLINE
| ID: mdl-35059924
ABSTRACT
Long non-coding RNAs (lncRNAs) are a type of non-coding RNAs with transcript lengths exceeding 200 nt. lncRNAs suppress or promote cancer mainly by regulating gene expression. The aim of this study was to explore the role of lncRNAs activated in metastatic PCa (lncAMPC) in nasopharyngeal carcinoma (NPC). Total RNAs were isolated from NPC and adjacent non-tumor tissues from 60 NPC patients and subjected to RT-qPCR to analyze differential expression of lncAMPC and miR-214. The roles of lncAMPC and miR-214 in regulating PTEN expression were analyzed using RT-qPCR and Western blot. Cell proliferation was analyzed using the BrdU assay. The results showed that lncAMPC was overexpressed in NPC tissues and was localized in both nuclei and cytoplasms of NPC cells. MiR-214 was positively correlated with lncAMPC. LncAMPC overexpression upregulated miR-214 and indirectly downregulated PTEN via miR-214. BrdU incorporation assay showed that lncAMPC and miR-214 overexpression increased cell proliferation. PTEN overexpression completely reversed the promoting effects of lncAMPC and miR-214 overexpression on cell proliferation. Therefore, lncAMPC might downregulate PTEN via miR-214 to increase NPC cell proliferation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ARN Neoplásico
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Regulación hacia Abajo
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Regulación Enzimológica de la Expresión Génica
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Regulación Neoplásica de la Expresión Génica
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Neoplasias Nasofaríngeas
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MicroARNs
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Fosfohidrolasa PTEN
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ARN Largo no Codificante
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Carcinoma Nasofaríngeo
Límite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Mol Cell Biochem
Año:
2022
Tipo del documento:
Article