Characterization of large intact protein ions by mass spectrometry: What directions should we follow?
Biochim Biophys Acta Proteins Proteom
; 1870(4): 140758, 2022 04 01.
Article
en En
| MEDLINE
| ID: mdl-35077914
ABSTRACT
Theoretically, the gas-phase interrogation of whole proteoforms via mass spectrometry, known as top-down proteomics, bypasses the protein inference problem that afflicts peptide-centric proteomic approaches. Despite this obvious advantage, the application of top-down proteomics remains rare, mainly due to limited throughput and difficulty of analyzing proteins >30 kDa. Here we will discuss some of the problems encountered during the characterization of large proteoforms, and guided by a combination of theoretical background and experimental evidence we will describe some innovative data acquisition strategies and novel mass spectrometry technologies that can at least partially overcome such limitations.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas
/
Proteómica
Idioma:
En
Revista:
Biochim Biophys Acta Proteins Proteom
Año:
2022
Tipo del documento:
Article