Your browser doesn't support javascript.
loading
Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants.
Welch, Nicole L; Zhu, Meilin; Hua, Catherine; Weller, Juliane; Mirhashemi, Marzieh Ezzaty; Nguyen, Tien G; Mantena, Sreekar; Bauer, Matthew R; Shaw, Bennett M; Ackerman, Cheri M; Thakku, Sri Gowtham; Tse, Megan W; Kehe, Jared; Uwera, Marie-Martine; Eversley, Jacqueline S; Bielwaski, Derek A; McGrath, Graham; Braidt, Joseph; Johnson, Jeremy; Cerrato, Felecia; Moreno, Gage K; Krasilnikova, Lydia A; Petros, Brittany A; Gionet, Gabrielle L; King, Ewa; Huard, Richard C; Jalbert, Samantha K; Cleary, Michael L; Fitzgerald, Nicholas A; Gabriel, Stacey B; Gallagher, Glen R; Smole, Sandra C; Madoff, Lawrence C; Brown, Catherine M; Keller, Matthew W; Wilson, Malania M; Kirby, Marie K; Barnes, John R; Park, Daniel J; Siddle, Katherine J; Happi, Christian T; Hung, Deborah T; Springer, Michael; MacInnis, Bronwyn L; Lemieux, Jacob E; Rosenberg, Eric; Branda, John A; Blainey, Paul C; Sabeti, Pardis C; Myhrvold, Cameron.
Afiliación
  • Welch NL; Broad Institute of MIT and Harvard, Cambridge, MA, USA. nicole_welch@g.harvard.edu.
  • Zhu M; Harvard Program in Virology, Division of Medical Sciences, Harvard Medical School, Boston, MA, USA. nicole_welch@g.harvard.edu.
  • Hua C; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Weller J; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Mirhashemi ME; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Nguyen TG; Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.
  • Mantena S; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Bauer MR; Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.
  • Shaw BM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Ackerman CM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Thakku SG; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Tse MW; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Kehe J; Harvard Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA, USA.
  • Uwera MM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Eversley JS; Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.
  • Bielwaski DA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • McGrath G; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Braidt J; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Johnson J; Division of Health Sciences and Technology, Harvard Medical School and Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Cerrato F; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Moreno GK; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Krasilnikova LA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Petros BA; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Gionet GL; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • King E; Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.
  • Huard RC; Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.
  • Jalbert SK; Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.
  • Cleary ML; Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Boston, MA, USA.
  • Fitzgerald NA; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Gabriel SB; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Gallagher GR; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Smole SC; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Madoff LC; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
  • Brown CM; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Keller MW; Division of Health Sciences and Technology, Harvard Medical School and Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Wilson MM; Harvard/Massachusetts Institute of Technology MD-PhD Program, Harvard Medical School, Boston, MA, USA.
  • Kirby MK; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
  • Barnes JR; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Park DJ; State Health Laboratories, Rhode Island Department of Health, Providence, RI, USA.
  • Siddle KJ; State Health Laboratories, Rhode Island Department of Health, Providence, RI, USA.
  • Happi CT; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
  • Hung DT; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
  • Springer M; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • MacInnis BL; Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Lemieux JE; Massachusetts Department of Public Health, Boston, MA, USA.
  • Rosenberg E; Massachusetts Department of Public Health, Boston, MA, USA.
  • Branda JA; Massachusetts Department of Public Health, Boston, MA, USA.
  • Blainey PC; Massachusetts Department of Public Health, Boston, MA, USA.
  • Sabeti PC; Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
  • Myhrvold C; Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Nat Med ; 28(5): 1083-1094, 2022 05.
Article en En | MEDLINE | ID: mdl-35130561
ABSTRACT
The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear need for high-throughput, multiplexed and sensitive assays for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other respiratory viruses and their emerging variants. Here, we present a cost-effective virus and variant detection platform, called microfluidic Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (mCARMEN), which combines CRISPR-based diagnostics and microfluidics with a streamlined workflow for clinical use. We developed the mCARMEN respiratory virus panel to test for up to 21 viruses, including SARS-CoV-2, other coronaviruses and both influenza strains, and demonstrated its diagnostic-grade performance on 525 patient specimens in an academic setting and 166 specimens in a clinical setting. We further developed an mCARMEN panel to enable the identification of 6 SARS-CoV-2 variant lineages, including Delta and Omicron, and evaluated it on 2,088 patient specimens with near-perfect concordance to sequencing-based variant classification. Lastly, we implemented a combined Cas13 and Cas12 approach that enables quantitative measurement of SARS-CoV-2 and influenza A viral copies in samples. The mCARMEN platform enables high-throughput surveillance of multiple viruses and variants simultaneously, enabling rapid detection of SARS-CoV-2 variants.
Asunto(s)
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Gripe Humana / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Nat Med Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Gripe Humana / COVID-19 Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Nat Med Asunto de la revista: BIOLOGIA MOLECULAR / MEDICINA Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos