Phosphoproteomic Approaches to Evaluate ABA Signaling.
Methods Mol Biol
; 2462: 163-179, 2022.
Article
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| MEDLINE
| ID: mdl-35152388
ABSTRACT
Abscisic acid (ABA) is a major phytohormone that regulates various processes in plants (e.g., seed dormancy/germination, abiotic/biotic stress responses). As protein phosphorylation is involved in the major pathways of ABA signaling, it is necessary to elucidate the phosphosignaling pathway involved in the ABA response. Phosphoproteomics enables determination of the proteins phosphorylated in vivo, and recent studies have applied a comparative phosphoproteomic approach to analyze ABA signaling in plants. For example, ABA-responsive phosphoproteins were identified in barley embryos. Furthermore, a phosphoproteomic approach is useful for screening protein kinase substrates by comparative analysis using kinase knockout mutants. Here, some technical points regarding phosphoproteomic analyses of ABA responses in plants are described.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
/
Transducción de Señal
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2022
Tipo del documento:
Article
País de afiliación:
Japón