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miR-186-5p inhibits the progression of oral squamous cell carcinoma by targeting ITGA6 to impair the activity of the PI3K/AKT pathway.
Chen, Min; Zhang, Jing.
Afiliación
  • Chen M; Department of Oral Cavity, Affiliated Hospital of Jianghan University, The Sixth Hospital of Wuhan, Wuhan, China.
  • Zhang J; Department of Oral Cavity, Affiliated Hospital of Jianghan University, The Sixth Hospital of Wuhan, Wuhan, China.
J Oral Pathol Med ; 51(4): 322-331, 2022 Apr.
Article en En | MEDLINE | ID: mdl-35201653
BACKGROUND: microRNAs (miRNAs) are pivotal regulators of multiple biological processes. miR-186-5p functions as a tumor suppressor in a variety of cancers and promotes the malignant proliferation of oral squamous cell carcinoma (OSCC). This study aimed to clarify the role and regulatory mechanism of miR-186-5p in OSCC. METHODS: The levels of miR-186-5p and integrin subunit alpha 6 (ITGA6) were investigated in clinical specimens and OSCC cell lines by reverse transcription-quantitative polymerase chain reaction. The effects of miR-186-5p and ITGA6 on the cell migration, proliferation, and phosphatidylinositol 3-kinase (PI3K)/serine-threonine kinase (AKT) pathway activity were evaluated by transwell assay, cell counting kit 8 assay, and western blotting, respectively. A xenograft model was used to analyze the effect of miR-186-5p on tumor growth. Bioinformatic analyses were conducted to identify the putative targets of miR-186-5p in OSCC. RESULTS: Decreased miR-186-5p expression levels were observed in OSCC tumor tissues and cell lines. The overexpression of miR-186-5p suppressed the proliferation and migration of OSCC cells, and weakened the phosphorylation of PI3K and AKT. Moreover, the overexpression of miR-186-5p in xenograft tumor models impedes tumor growth. miR-186-5p is bound to ITGA6 and negatively related to ITGA6 expression in tumor tissues. The forced expression of ITGA6 promoted OSCC cell proliferation and migration and enhanced the phosphorylation levels of PI3K and AKT, while additional miR-186-5p enrichment partly abolished these effects. CONCLUSION: miR-186-5p binds to ITGA6 to impair the activity of the PI3K/AKT signaling pathway, thereby blocking the development of OSCC. This study provides insight to understand the pathogenesis of OSCC.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Carcinoma de Células Escamosas / MicroARNs / Neoplasias de Cabeza y Cuello Límite: Humans Idioma: En Revista: J Oral Pathol Med Asunto de la revista: ODONTOLOGIA / PATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Carcinoma de Células Escamosas / MicroARNs / Neoplasias de Cabeza y Cuello Límite: Humans Idioma: En Revista: J Oral Pathol Med Asunto de la revista: ODONTOLOGIA / PATOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: China