Identification of stabilizing point mutations through mutagenesis of destabilized protein libraries.

Ahmed, Shahbaz; Manjunath, Kavyashree; Chattopadhyay, Gopinath; Varadarajan, Raghavan

Ahmed, Shahbaz; Manjunath, Kavyashree; Chattopadhyay, Gopinath; Varadarajan, Raghavan.

Afiliación

Ahmed S; Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Manjunath K; Centre for Chemical Biology and Therapeutics, Institute of Stem Cell Science and Regenerative Medicine, Bangalore, India.
Chattopadhyay G; Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Varadarajan R; Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India. Electronic address: varadar@iisc.ac.in.

J Biol Chem ; 298(4): 101785, 2022 04.

Article en En | MEDLINE | ID: mdl-35247389

Asunto(s)

Mutación Puntual; Estabilidad Proteica; Proteínas; Mutagénesis; Mutación Puntual/genética; Proteínas/química; Saccharomyces cerevisiae/genética

Palabras clave

High-throughput screening (HTS); hyperstable mutants; protein engineering; protein evolution; protein folding; protein stability; protein structure; second-site suppressor mutagenesis

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas / Mutación Puntual / Estabilidad Proteica Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Biol Chem Año: 2022 Tipo del documento: Article País de afiliación: India

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google