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Patterning of the cell cortex and the localization of cleavage furrows in multi-nucleate cells.
Gerisch, Günther; Prassler, Jana; Ecke, Mary.
Afiliación
  • Gerisch G; Max Planck Institute of Biochemistry, Cell Dynamics Group, Am Klopferspitz 18, D-82152 Martinsried, Germany.
  • Prassler J; Max Planck Institute of Biochemistry, Cell Dynamics Group, Am Klopferspitz 18, D-82152 Martinsried, Germany.
  • Ecke M; Max Planck Institute of Biochemistry, Cell Dynamics Group, Am Klopferspitz 18, D-82152 Martinsried, Germany.
J Cell Sci ; 135(7)2022 04 01.
Article en En | MEDLINE | ID: mdl-35274133
In multi-nucleate cells of Dictyostelium, cytokinesis is performed by unilateral cleavage furrows that ingress the large cells from their border. We use a septase (sepA)-null mutant with delayed cytokinesis to show that in anaphase a pattern is generated in the cell cortex of cortexillin and myosin II. In multi-nucleate cells, these proteins decorate the entire cell cortex except circular zones around the centrosomes. Unilateral cleavage furrows are initiated at spaces free of microtubule asters and invade the cells along trails of cortexillin and myosin II accumulation. Where these areas widen, the cleavage furrow may branch or expand. When two furrows meet, they fuse, thus separating portions of the multi-nucleate cell from each other. Unilateral furrows are distinguished from the contractile ring of a normal furrow by their expansion rather than constriction. This is particularly evident for expanding ring-shaped furrows that are formed in the centre of a large multi-nucleate cell. Our data suggest that the myosin II-enriched area in multi-nucleate cells is a contractile sheet that pulls on the unilateral furrows and, in that way, expands them.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Dictyostelium Idioma: En Revista: J Cell Sci Año: 2022 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Dictyostelium Idioma: En Revista: J Cell Sci Año: 2022 Tipo del documento: Article País de afiliación: Alemania