In-vitro and in-vivo biocompatibility of dECM-alginate as a promising candidate in cell delivery for kidney regeneration.

ABSTRACT

In this study, kidney decellularized extracellular matrix (dECM) and alginate (ALG) hybrid injectable hydrogel, with the purpose of delivering progenitor cells for tissue engineering, were prepared by using a physical crosslinking method in a CaCl2 solution with high porosity for the exchange of nutrition and waste. In addition, the physical appearance and surface morphology of the hydrogel were investigated using optical and scanning electron microscopy, respectively. The functional groups of the dECM/ALG xerogels was examined via Fourier transform infrared spectroscopy. The biocompatibility of dECM/ALG xerogels was examined in-vitro using renal progenitor cells obtained from adult rat kidney. Enhanced biocompatibility and significant hemostatic behavior was noticed. Furthermore, the in-vivo biocompatibility of dECM/ALG hydrogel with progenitor cells was determined in the deep renal cortex for 7 and 21 days, in order to assess the foreign body reaction and inflammatory response. Early-stage glomerulus-like structure and dense linear cell network-like phenomenon were noticed. Loading of progenitor cells together with hydrogel enhances the cell density obviously due to cell migration from host and form a pattern. The desired early stage in-vivo response to progenitor cell-laden dECM/ALG hydrogel plays a potential role in kidney regeneration long term.