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Adaptation of the gut pathobiont Enterococcus faecalis to deoxycholate and taurocholate bile acids.
Repoila, F; Le Bohec, F; Guérin, C; Lacoux, C; Tiwari, S; Jaiswal, A K; Santana, M Passos; Kennedy, S P; Quinquis, B; Rainteau, D; Juillard, V; Furlan, S; Bouloc, P; Nicolas, P; Miyoshi, A; Azevedo, V; Serror, P.
Afiliación
  • Repoila F; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France. francis.repoila@inrae.fr.
  • Le Bohec F; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
  • Guérin C; Université de Strasbourg, INRAE, SVQV UMR-A 1131, 68000, Colmar, France.
  • Lacoux C; Université Paris-Saclay, INRAE, MaIAGE, 78350, Jouy-en-Josas, France.
  • Tiwari S; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
  • Jaiswal AK; ImmunoSearch, Les Cyclades, Chemin de Camperousse, 06130, Grasse, France.
  • Santana MP; Universidade Federal de Minas Gerais, ICB/UFMG, Minas Gerais, Belo Horizonte, 31270-901, Brazil.
  • Kennedy SP; Universidade Federal de Minas Gerais, ICB/UFMG, Minas Gerais, Belo Horizonte, 31270-901, Brazil.
  • Quinquis B; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
  • Rainteau D; Universidade Federal de Minas Gerais, ICB/UFMG, Minas Gerais, Belo Horizonte, 31270-901, Brazil.
  • Juillard V; Department of Computational Biology, USR3756 CNRS, Institut Pasteur, Paris, France.
  • Furlan S; Université Paris-Saclay, INRAE, MGP, 78350, Jouy-en-Josas, France.
  • Bouloc P; Sorbonne Université, Inserm, Centre de Recherche Saint-Antoine, CRSA, AP-HP, Hôpital Saint Antoine, 75012, Paris, France.
  • Nicolas P; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
  • Miyoshi A; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
  • Azevedo V; Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.
  • Serror P; Université Paris-Saclay, INRAE, MaIAGE, 78350, Jouy-en-Josas, France.
Sci Rep ; 12(1): 8485, 2022 05 19.
Article en En | MEDLINE | ID: mdl-35590028
Enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. This bacterial species is subdominant in a healthy physiological state of the gut microbiota (eubiosis) in adults, but can become dominant and cause infections when the intestinal homeostasis is disrupted (dysbiosis). The relatively high concentrations of bile acids deoxycholate (DCA) and taurocholate (TCA) hallmark eubiosis and dysbiosis, respectively. This study aimed to better understand how E. faecalis adapts to DCA and TCA. We showed that DCA impairs E. faecalis growth and possibly imposes a continuous adjustment in the expression of many essential genes, including a majority of ribosomal proteins. This may account for slow growth and low levels of E. faecalis in the gut. In contrast, TCA had no detectable growth effect. The evolving transcriptome upon TCA adaptation showed the early activation of an oligopeptide permease system (opp2) followed by the adjustment of amino acid and nucleotide metabolisms. We provide evidence that TCA favors the exploitation of oligopeptide resources to fuel amino acid needs in limiting oligopeptide conditions. Altogether, our data suggest that the combined effects of decreased DCA and increased TCA concentrations can contribute to the rise of E. faecalis population during dysbiosis.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ácidos y Sales Biliares / Enterococcus faecalis Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ácidos y Sales Biliares / Enterococcus faecalis Límite: Humans Idioma: En Revista: Sci Rep Año: 2022 Tipo del documento: Article País de afiliación: Francia