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Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single-Cell RNA Sequencing.
Hen-Avivi, Shelly; Avraham, Roi.
Afiliación
  • Hen-Avivi S; Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel.
  • Avraham R; Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel. roi.avraham@weizmann.ac.il.
Methods Mol Biol ; 2427: 133-147, 2022.
Article en En | MEDLINE | ID: mdl-35619031
Complex interactions between diverse host immune cells can determine the outcome of pathogen infections. Advances in single-cell RNA sequencing (scRNA-seq) allow detection of the transcriptional patterns of different immune cells at steady state and after infection. To reveal the complex interactions of the human immune system in response to diverse intracellular pathogens, we developed a protocol for scRNA-seq of ex vivo infected human peripheral blood mononuclear cells (PBMCs). We demonstrate here infection with Salmonella enterica serovar Typhimurium, but this protocol can be used for any other pathogen of interest, and expand our knowledge of human host-pathogen biology.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Interacciones Huésped-Patógeno Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Israel

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Leucocitos Mononucleares / Interacciones Huésped-Patógeno Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2022 Tipo del documento: Article País de afiliación: Israel