Labeling of sarcoma associated monoclonal antibody with 111In, 67Ga and 125I.
Radiother Oncol
; 8(2): 129-35, 1987 Feb.
Article
en En
| MEDLINE
| ID: mdl-3562892
ABSTRACT
Labeling of human sarcoma-associated murine monoclonal antibody (MAb) 23H7 with 67Ga and 111In by the bifunctional ligand method is reported. 67Ga was chelated to the MAb via desferrioxamine B and 111In via the cyclic anhydride of DTPA. Higher specific activity was obtained with 67Ga (4-5 microCi/micrograms) as compared with 111In (2 microCi/micrograms). The binding capacity of the MAb was confirmed by repeated indirect immuno-fluorescence assays performed before and after labeling. A fast blood clearance was observed 33% recovered dose (R.D.) blood level 3 h post-injection as compared with 56% after injection of control polyclonal IgG. Preliminary results on chemically induced sarcoma bearing mice showed a relatively high tumor uptake of the labeled antibody.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Radioisótopos
/
Sarcoma
/
Radioisótopos de Galio
/
Indio
/
Radioisótopos de Yodo
/
Marcaje Isotópico
/
Anticuerpos Monoclonales
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Radiother Oncol
Año:
1987
Tipo del documento:
Article