CRISPR-Guided Proximity Labeling of RNA-Protein Interactions.
Genes (Basel)
; 13(9)2022 08 27.
Article
en En
| MEDLINE
| ID: mdl-36140717
ABSTRACT
Proximity labeling employs modified biotin ligases or peroxidases that produce reactive radicals to covalently label proximate proteins with biotin in living cells. The resulting biotinylated proteins can then be isolated and identified. A combination of programmable DNA targeting and proximity labeling that maps proteomic landscape at DNA elements with dCas9-APEX2 has been established in living cells. However, defining interactome at RNA elements has lagged behind. In combination with RNA-targeting CRISPR-Cas13, proximity labeling can also be used to identify proteins that interact with specific RNA elements in living cells. From this viewpoint, we briefly summarize the latest advances in CRISPR-guided proximity labeling in studying RNA-protein interactions, and we propose applying the most recent engineered proximity-labeling enzymes to study RNA-centric interactions in the future.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Biotina
/
Proteómica
Idioma:
En
Revista:
Genes (Basel)
Año:
2022
Tipo del documento:
Article
País de afiliación:
China