Evaluation of Bactericidal Effects of H2- and O3-filled Ultrafine Bubbles Water.

Ultrafine bubbles (UFBs) are gaining attention in diverse industries as a new type of material with specific physical properties. Bactericidal activity has been reported as one of the unique properties of UFB water; however, the bactericidal activities of UFBs related to the gas type remain unclear. In particular, the bactericidal effect of hydrogen (H2) -filled UFB water has not been verified. Therefore, this study aimed to evaluate the bactericidal effects of H2- or ozone (O3) -filled UFB water using a bacterial suspension test. The results of this study clearly showed that H2- or O3-filled UFB water had strong bactericidal activity. Exposure of Escherichia coli for 6 h and Staphylococcus aureus for 3 h reduced the survival rate of those bacteria by >90%. This finding suggests that both O3 gas- and H2-filled UFBs are novel environmentally friendly disinfectants that can be employed to avoid the use of chemicals.