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Differential expression of inflammatory cytokines, prostaglandin synthases and secretory leukocyte protease inhibitor in the endometrium and circulation in different graded CEH-pyometra in bitch.
Sasidharan, J K; Patra, M K; Khan, J A; Singh, A K; Karikalan, M; De, U K; Saxena, A C; Dubal, Z B; Singh, S K; Kumar, H; Krishnaswamy, N.
Afiliación
  • Sasidharan JK; Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Patra MK; Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India. Electronic address: drmanas01@gmail.com.
  • Khan JA; Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Singh AK; Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Karikalan M; Centre for Wildlife Conservation, Management and Disease Surveillance, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • De UK; Division of Veterinary Medicine, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India; Referral Veterinary Polyclinic, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Saxena AC; Referral Veterinary Polyclinic, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Dubal ZB; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Singh SK; Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Kumar H; Animal Reproduction Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India.
  • Krishnaswamy N; Indian Veterinary Research Institute, Hebbal, Bengaluru, 560 024, India.
Theriogenology ; 197: 139-149, 2023 Feb.
Article en En | MEDLINE | ID: mdl-36516536
Cystic endometrial hyperplasia (CEH)-pyometra (CEH-P) is one of the most common reproductive disorders in bitches, posing a risk to both future fertility and life. The aims of the current study were to elucidate the differential expression patterns of inflammatory mediators at transcript and protein levels in the endometrium and to assess the concentrations of key inflammatory mediators in the peripheral circulation of bitches with different graded CEH-P. A total of 25 client-owned intact mixed breed bitches of 3-10 years presented to the outpatient department of RVP-TVCC of the institute were considered for the study. Of which, 22 cases suggestive of pyometra and 3 cases of CEH obtained during routine elective ovariohysterectomy were subjected to histopathological examination. Uteri were categorized into CEH (n = 3), moderate CEH-P (mCEH-P, n = 9), severe CEH-P (sCEH-P, n = 6) and atrophic pyometra (AT-P, n = 7). A group of age matched (n = 12) bitches without pyometra served as control. Endometrial transcripts such as IL6, IL8, PTGS2, PGFS, and SLPI were expressed differentially in the CEH and CEH-P bitch. In addition, a strong immunoreactivity (IR) of IL6, IL8, PTGS2, and mPGES1 was recorded in the sCEH-P uterus, while expression of IL10 was noticed in AT-P. In circulation, serum IL6 was the most relevant marker with high sensitivity of 96.2% and specificity of 84.6% at a cut off concentration 8.5 pg/mL followed by SLPI with 95.2% sensitivity, and 84.6% specificity at cut off concentration of 1.3 ng/mL. Serum IL10, PGFM and SLPI concentration in the peripheral circulation were 1.5-2.23 fold higher in mCEH-P, 0.87-2.5 fold higher in sCEH-P and 2.9-3.5 fold higher in AT-P than that of control. It is concluded that monitoring the serum concentration of IL6, IL10 and SLPI would be useful adjunct to the established hematobiochemical parameters in the management of pyometra in the bitch with critical illness.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de los Perros / Hiperplasia Endometrial / Piómetra Límite: Animals Idioma: En Revista: Theriogenology Año: 2023 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de los Perros / Hiperplasia Endometrial / Piómetra Límite: Animals Idioma: En Revista: Theriogenology Año: 2023 Tipo del documento: Article País de afiliación: India