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Engineering Geobacter pili to produce metal:organic filaments.
Szmuc, Eric; Walker, David J F; Kireev, Dmitry; Akinwande, Deji; Lovley, Derek R; Keitz, Benjamin; Ellington, Andrew.
Afiliación
  • Szmuc E; College of Natural Sciences, University of Texas at Austin, Austin, TX, 78712, United States.
  • Walker DJF; College of Natural Sciences, University of Texas at Austin, Austin, TX, 78712, United States; U.S. Army Engineer Research and Development Center, Environmental Laboratory, University of Texas at Austin, Austin, TX, 78712, United States; Bioconscientia LLC, Austin, TX 78712, United States.
  • Kireev D; Cockrell School of Engineering, University of Texas at Austin, Austin, TX, 78712, United States.
  • Akinwande D; Cockrell School of Engineering, University of Texas at Austin, Austin, TX, 78712, United States.
  • Lovley DR; Department of Microbiology, University of Massachusetts-Amherst, Amherst, MA, 01003, United States.
  • Keitz B; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, 78712, United States.
  • Ellington A; College of Natural Sciences, University of Texas at Austin, Austin, TX, 78712, United States. Electronic address: andy.ellington@austin.utexas.edu.
Biosens Bioelectron ; 222: 114993, 2023 Feb 15.
Article en En | MEDLINE | ID: mdl-36525710
The organized self-assembly of conductive biological structures holds promise for creating new bioelectronic devices. In particular, Geobacter sulfurreducens type IVa pili have proven to be a versatile material for fabricating protein nanowire-based devices. To scale the production of conductive pili, we designed a strain of Shewanella oneidensis that heterologously expressed abundant, conductive Geobacter pili when grown aerobically in liquid culture. S. oneidensis expressing a cysteine-modified pilin, designed to enhance the capability to bind to gold, generated conductive pili that self-assembled into biohybrid filaments in the presence of gold nanoparticles. Elemental composition analysis confirmed the filament-metal interactions within the structures, which were several orders of magnitude larger than previously described metal:organic filaments. The results demonstrate that the S. oneidensis chassis significantly advances the possibilities for facile conductive protein nanowire design and fabrication.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Geobacter / Nanopartículas del Metal Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Geobacter / Nanopartículas del Metal Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos