An immunocompetent rectal cancer model to study radiation therapy.
Cell Rep Methods
; 2(12): 100353, 2022 12 19.
Article
en En
| MEDLINE
| ID: mdl-36590695
ABSTRACT
We describe a mouse model of rectal cancer (RC) involving rapid tumor organoid engraftment via orthotopic transplantation in an immunocompetent setting. This approach uses simple mechanical disruption to allow engraftment, avoiding the use of dextran sulfate sodium. The resulting RC tumors invaded from the mucosal surface and metastasized to distant organs. Histologically, the tumors closely resemble human RC and mirror remodeling of the tumor microenvironment in response to radiation. This murine RC model thus recapitulates key aspects of human RC pathogenesis and presents an accessible approach for more physiologically accurate, preclinical efficacy studies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Neoplasias del Recto
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell Rep Methods
Año:
2022
Tipo del documento:
Article
País de afiliación:
Estados Unidos