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Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9.
Guo, Ruirui; Yuan, Simin; Li, Bing; Wang, Jichang; Sun, Chuanbo.
Afiliación
  • Guo R; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510275, China.
  • Yuan S; Department of Prenatal Diagnostic Center, Guangzhou Women and Children's Medical Center, China.
  • Li B; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510275, China.
  • Wang J; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510275, China.
  • Sun C; Laboratory of Medical Systems Biology, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangdong Provincial Clinical Research Center for Child Health, Guangzhou 510623, China. Electronic address: bobo_1110@163.com.
Stem Cell Res ; 67: 103022, 2023 03.
Article en En | MEDLINE | ID: mdl-36630838
NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Human primordial germ cell-like cells (hPGCLCs) generated from pluripotent stem cells is an excellent alternatives in human germ cell-related studies. This hESC line with an mCherry knock-in at the site before NANOS3's stop codon serves as a useful tool to learn human PGC specification.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Madre Embrionarias Humanas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2023 Tipo del documento: Article País de afiliación: China
Texto completo
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XML
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Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Células Madre Embrionarias Humanas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2023 Tipo del documento: Article País de afiliación: China