Mass spectrometry imaging-based assays for aminotransferase activity reveal a broad substrate spectrum for a previously uncharacterized enzyme.

de Raad, Markus; Koper, Kaan; Deng, Kai; Bowen, Benjamin P; Maeda, Hiroshi A; Northen, Trent R

de Raad, Markus; Koper, Kaan; Deng, Kai; Bowen, Benjamin P; Maeda, Hiroshi A; Northen, Trent R.

Afiliación

de Raad M; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA. Electronic address: MdeRaad@lbl.gov.
Koper K; Department of Botany, University of Wisconsin-Madison; Madison, Wisconsin, USA.
Deng K; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California, USA; Sandia National Laboratories, Livermore, California, USA.
Bowen BP; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA; Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Maeda HA; Department of Botany, University of Wisconsin-Madison; Madison, Wisconsin, USA.
Northen TR; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA; Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, California, USA; Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, California, U

J Biol Chem ; 299(3): 102939, 2023 03.

Article en En | MEDLINE | ID: mdl-36702250

Asunto(s)

Aminoácidos; Pruebas de Enzimas; Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción; Transaminasas; Aminoácidos/metabolismo; Especificidad por Sustrato; Transaminasas/química; Transaminasas/metabolismo; Pruebas de Enzimas/métodos; Arabidopsis/enzimología

Palabras clave

aminotransferases; enzyme promiscuity; enzyme screening; high-throughput screening; mass spectrometry; mass spectrometry imaging; nanostructure-initiator mass spectrometry; pyridoxal-5'-phosphatedependent enzyme

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción / Pruebas de Enzimas / Aminoácidos / Transaminasas Idioma: En Revista: J Biol Chem Año: 2023 Tipo del documento: Article

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