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LINE-1 ORF1p as a candidate biomarker in high grade serous ovarian carcinoma.
Sato, Sho; Gillette, Michael; de Santiago, Pamela R; Kuhn, Eric; Burgess, Michael; Doucette, Kristen; Feng, Yi; Mendez-Dorantes, Carlos; Ippoliti, Paul J; Hobday, Sara; Mitchell, Marilyn A; Doberstein, Kai; Gysler, Stefan M; Hirsch, Michelle S; Schwartz, Lauren; Birrer, Michael J; Skates, Steven J; Burns, Kathleen H; Carr, Steven A; Drapkin, Ronny.
Afiliación
  • Sato S; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Gillette M; The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
  • de Santiago PR; Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital, Boston, MA, 02114, USA.
  • Kuhn E; Harvard Medical School, Boston, MA, 02115, USA.
  • Burgess M; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Doucette K; The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
  • Feng Y; The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
  • Mendez-Dorantes C; The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
  • Ippoliti PJ; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Hobday S; Department of Oncologic Pathology, Dana-Farber Cancer Institute, Boston, MA, USA.
  • Mitchell MA; The Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.
  • Doberstein K; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Gysler SM; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Hirsch MS; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Schwartz L; Penn Ovarian Cancer Research Center, University of Pennsylvania, Perelman School of Medicine, Philadelphia, PA, 19104, USA.
  • Birrer MJ; Department of Pathology, Brigham and Women's Hospital, Boston, MA, 02115, USA.
  • Skates SJ; Harvard Medical School, Boston, MA, 02115, USA.
  • Burns KH; Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, PA, 19104, USA.
  • Carr SA; Department of Medicine, University of Alabama at Birmingham, Birmingham, AL, 35233, USA.
  • Drapkin R; Harvard Medical School, Boston, MA, 02115, USA.
Sci Rep ; 13(1): 1537, 2023 01 27.
Article en En | MEDLINE | ID: mdl-36707610
ABSTRACT
Long interspersed element 1 (LINE-1) open reading frame 1 protein (ORF1p) expression is a common feature of many cancer types, including high-grade serous ovarian carcinoma (HGSOC). Here, we report that ORF1p is not only expressed but also released by ovarian cancer and primary tumor cells. Immuno-multiple reaction monitoring-mass spectrometry assays showed that released ORF1p is confidently detectable in conditioned media, ascites, and patients' plasma, implicating ORF1p as a potential biomarker. Interestingly, ORF1p expression is detectable in fallopian tube (FT) epithelial precursors of HGSOC but not in benign FT, suggesting that ORF1p expression in an early event in HGSOC development. Finally, treatment of FT cells with DNA methyltransferase inhibitors led to robust expression and release of ORF1p, validating the regulatory role of DNA methylation in LINE-1 repression in non-tumorigenic tissue.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias Ováricas Límite: Female / Humans Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neoplasias Ováricas Límite: Female / Humans Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos