Comprehensive Surface Histology of Fresh Resection Margins With Rapid Open-Top Light-Sheet (OTLS) Microscopy.
IEEE Trans Biomed Eng
; 70(7): 2160-2171, 2023 07.
Article
en En
| MEDLINE
| ID: mdl-37021859
ABSTRACT
OBJECTIVE:
For tumor resections, margin status typically correlates with patient survival but positive margin rates are generally high (up to 45% for head and neck cancer). Frozen section analysis (FSA) is often used to intraoperatively assess the margins of excised tissue, but suffers from severe under-sampling of the actual margin surface, inferior image quality, slow turnaround, and tissue destructiveness.METHODS:
Here, we have developed an imaging workflow to generate en face histologic images of freshly excised surgical margin surfaces based on open-top light-sheet (OTLS) microscopy. Key innovations include (1) the ability to generate false-colored H&E-mimicking images of tissue surfaces stained for < 1 min with a single fluorophore, (2) rapid OTLS surface imaging at a rate of 15 min/cm2 followed by real-time post-processing of datasets within RAM at a rate of 5 min/cm2, and (3) rapid digital surface extraction to account for topological irregularities at the tissue surface.RESULTS:
In addition to the performance metrics listed above, we show that the image quality generated by our rapid surface-histology method approaches that of gold-standard archival histology.CONCLUSION:
OTLS microscopy has the feasibility to provide intraoperative guidance of surgical oncology procedures.SIGNIFICANCE:
The reported methods can potentially improve tumor-resection procedures, thereby improving patient outcomes and quality of life.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Márgenes de Escisión
/
Microscopía
Límite:
Humans
Idioma:
En
Revista:
IEEE Trans Biomed Eng
Año:
2023
Tipo del documento:
Article