Your browser doesn't support javascript.
loading
METTL3 affects FLT3-ITD+ acute myeloid leukemia by mediating autophagy by regulating PSMA3-AS1 stability.
Wu, Shenghao; Weng, Shanshan; Zhou, Wenjin; Chen, Yuemiao; Liu, Zhen.
Afiliación
  • Wu S; Department of Hematology, The Dingli Clinical Institute of Wenzhou Medical University (The Second Affiliated Hospital of Shanghai University , Wenzhou Central Hospital), Wenzhou, Zhejiang Province, China.
  • Weng S; Department of Hematology, The Dingli Clinical Institute of Wenzhou Medical University (The Second Affiliated Hospital of Shanghai University , Wenzhou Central Hospital), Wenzhou, Zhejiang Province, China.
  • Zhou W; Department of Hematology, The Dingli Clinical Institute of Wenzhou Medical University (The Second Affiliated Hospital of Shanghai University , Wenzhou Central Hospital), Wenzhou, Zhejiang Province, China.
  • Chen Y; Department of Hematology, The Dingli Clinical Institute of Wenzhou Medical University (The Second Affiliated Hospital of Shanghai University , Wenzhou Central Hospital), Wenzhou, Zhejiang Province, China.
  • Liu Z; Department of Hematology, The Dingli Clinical Institute of Wenzhou Medical University (The Second Affiliated Hospital of Shanghai University , Wenzhou Central Hospital), Wenzhou, Zhejiang Province, China.
Cell Cycle ; 22(10): 1232-1245, 2023 05.
Article en En | MEDLINE | ID: mdl-37088992
ABSTRACT
The study was designed to explore the role of PSMA3-AS1 in initiation and progression of acute myeloid leukemia (AML) and investigate its action mechanism. Expression of PSMA3-AS1, miR-20a-5p and ATG16L1 both in vitro and in vivo was measured by qRT-PCR. The expression of protein was detected by western blot assay. Edu staining and flow cytometry were utilized to measure cell proliferation and apoptosis. Potential target was predicted by bioinformatics and was verified by dual-luciferase report gene assay and RNA pull down assay. QRT-PCR was used to quantify autophagy (LC3, Beclin1, P62) related genes. The m6A modification test is used to verify the effect of METTL3 on PSMA3-AS1. Tumor model was used to identify the effect of PSMA3-AS1 on tumor growth in vivo, and immunohistochemistry was applied to detect expression of ki67 and TUNEL. The results indicate that PSMA3-AS1 was upregulated in FLT3-ITD+ AML patients. Si-PSMA3-AS1 could inhibit the proliferation, autophagy and promote the apoptosis in MV4-11 and Molm13 cells. METTL3 could enhance the PSMA3-AS1 RNA stability. In addition, this study revealed that PSMA3-AS1 affected FLT3-ITD+ AML by targeting expression of miR-20a-5p, and miR-20a-5p further modulated expression of ATG16L1, an mRNA that down-regulated in AML, to affect disease advancement. PSMA3-AS1 could promote FLT3-ITD+ AML progression by regulating the level of autophagy through miR-20a-5p/ATG16L1 pathway. In addition, the increase of PSMA3-AS1 may be caused by the involvement of METTL3 in regulating its stability. This discovery will provide new horizons for early screening and targeted therapy of FLT3-ITD+ AML.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / MicroARNs Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cell Cycle Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / MicroARNs Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cell Cycle Año: 2023 Tipo del documento: Article País de afiliación: China