Characteristics and expression profiles of MHC class â
molecules in Carassius auratus.
Fish Shellfish Immunol
; 137: 108794, 2023 Jun.
Article
en En
| MEDLINE
| ID: mdl-37146848
ABSTRACT
Major histocompatibility complex class â
(MHC â
) molecules play a vital role in adaptive immune systems in vertebrates by presenting antigens to effector T cells. Understanding the expression profiling of MHC â
molecules in fish is essential for improving our knowledge of the relationship between microbial infection and adaptive immunity. In this study, we conducted a comprehensive analysis of MHC â
gene characteristics in Carassius auratus, an important freshwater aquaculture fish in China that is susceptible to Cyprinid herpesvirus 2 (CyHV-2) infection. We identified approximately 20 MHC â
genes discussed, including U, Z, and L lineage genes. However, only U and Z lineage proteins were identified in the kidney of Carassius auratus using high pH reversed-phase chromatography and mass spectrometry. The L lineage proteins were either not expressed or present at an extremely low level in the kidneys of Carassius auratus. We also used targeted proteomics to analyze changes in protein MHC â
molecules abundance in healthy and CyHV-2-infected Carassius auratus. We observed that five MHC â
molecules were upregulated, and Caau-UFA was downregulated in the diseased group. This study is the first to reveal the expression of MHC â
molecules at a large scale in Cyprinids, which enhances our understanding of fish adaptive immune systems.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infecciones por Herpesviridae
/
Enfermedades de los Peces
/
Herpesviridae
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Fish Shellfish Immunol
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
China