N4-acetylation of cytidine in mRNA plays essential roles in plants.
Plant Cell
; 35(10): 3739-3756, 2023 09 27.
Article
en En
| MEDLINE
| ID: mdl-37367221
ABSTRACT
The biological function of RNA can be modulated by base modifications. Here, we unveiled the occurrence of N4-acetylation of cytidine in plant RNA, including mRNA, by employing LC-MS/MS and acRIP-seq. We identified 325 acetylated transcripts from the leaves of 4-week-old Arabidopsis (Arabidopsis thaliana) plants and determined that 2 partially redundant N-ACETYLTRANSFERASEs FOR CYTIDINE IN RNA (ACYR1 and ACYR2), which are homologous to mammalian NAT10, are required for acetylating RNA in vivo. A double-null mutant was embryo lethal, while eliminating 3 of the 4 ACYR alleles led to defects in leaf development. These phenotypes could be traced back to the reduced acetylation and concomitant destabilization of the transcript of TOUGH, which is required for miRNA processing. These findings indicate that N4-acetylation of cytidine is a modulator of RNA function with a critical role in plant development and likely many other processes.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Arabidopsis
/
Citidina
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Plant Cell
Asunto de la revista:
BOTANICA
Año:
2023
Tipo del documento:
Article