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Sub-1.4 cm3 capsule for detecting labile inflammatory biomarkers in situ.
Inda-Webb, M E; Jimenez, M; Liu, Q; Phan, N V; Ahn, J; Steiger, C; Wentworth, A; Riaz, A; Zirtiloglu, T; Wong, K; Ishida, K; Fabian, N; Jenkins, J; Kuosmanen, J; Madani, W; McNally, R; Lai, Y; Hayward, A; Mimee, M; Nadeau, P; Chandrakasan, A P; Traverso, G; Yazicigil, R T; Lu, T K.
Afiliación
  • Inda-Webb ME; Synthetic Biology Group, MIT Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Jimenez M; Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Liu Q; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Phan NV; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Ahn J; Division of Gastroenterology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Steiger C; Electrical and Computer Engineering Department, Boston University, Boston, MA, USA.
  • Wentworth A; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Riaz A; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Zirtiloglu T; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Wong K; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Ishida K; Division of Gastroenterology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Fabian N; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Jenkins J; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Kuosmanen J; Division of Gastroenterology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Madani W; Electrical and Computer Engineering Department, Boston University, Boston, MA, USA.
  • McNally R; Electrical and Computer Engineering Department, Boston University, Boston, MA, USA.
  • Lai Y; Division of Gastroenterology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Hayward A; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Mimee M; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Nadeau P; Division of Gastroenterology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Chandrakasan AP; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Traverso G; The Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Yazicigil RT; Division of Comparative Medicine, MIT, Cambridge, MA, USA.
  • Lu TK; Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nature ; 620(7973): 386-392, 2023 Aug.
Article en En | MEDLINE | ID: mdl-37495692
Transient molecules in the gastrointestinal tract such as nitric oxide and hydrogen sulfide are key signals and mediators of inflammation. Owing to their highly reactive nature and extremely short lifetime in the body, these molecules are difficult to detect. Here we develop a miniaturized device that integrates genetically engineered probiotic biosensors with a custom-designed photodetector and readout chip to track these molecules in the gastrointestinal tract. Leveraging the molecular specificity of living sensors1, we genetically encoded bacteria to respond to inflammation-associated molecules by producing luminescence. Low-power electronic readout circuits2 integrated into the device convert the light emitted by the encapsulated bacteria to a wireless signal. We demonstrate in vivo biosensor monitoring in the gastrointestinal tract of small and large animal models and the integration of all components into a sub-1.4 cm3 form factor that is compatible with ingestion and capable of supporting wireless communication. With this device, diseases such as inflammatory bowel disease could be diagnosed earlier than is currently possible, and disease progression could be more accurately tracked. The wireless detection of short-lived, disease-associated molecules with our device could also support timely communication between patients and caregivers, as well as remote personalized care.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Biomarcadores / Técnicas Biosensibles / Sulfuro de Hidrógeno / Inflamación / Óxido Nítrico Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Nature Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Biomarcadores / Técnicas Biosensibles / Sulfuro de Hidrógeno / Inflamación / Óxido Nítrico Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Nature Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos