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Establishment of a Model for Human Hypertrophic Scar Using Tissue Engineering Method.
Li, Yawei; Shan, Xiaofeng; Liang, Jie; Cai, Zhigang.
Afiliación
  • Li Y; Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing.
  • Shan X; Department of Oral & Maxillofacial Surgery and Oral Biomedical Engineering Laboratory Shanghai Stomatological Hospital Fudan University, Shanghai, China.
  • Liang J; Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing.
  • Cai Z; Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing.
J Craniofac Surg ; 35(1): 268-272, 2024.
Article en En | MEDLINE | ID: mdl-37602502
ABSTRACT

BACKGROUND:

Treatment of human hypertrophic scar (HS) is a challenge for plastic surgeons, whereas the clinical and experimental research has been limited due to the lack of an ideal model of human HS tissue.

OBJECTIVE:

To establish a model of human HS using tissue engineering method, to improve the research for HS in the clinic and laboratory.

METHODS:

Hypertrophic scar fibroblasts (HSFBs) were transferred to polylactic acid (PLA)/polyglycolic acid (PGA) scaffolds. Biocompatibility of HSFBs-PLA/PGA composites was evaluated using scanning electron microscopy. Composites of HSFBs-PLA/PGA were implanted in subcutaneous pockets in athymic mice after 4 weeks in vitro culture. A re-entry operation was performed to obtain the HS-like tissues after 12 weeks of in vivo culture. The histological stain, the expression of type I collagen, the proliferation ability, and vitality of HSFBs were compared between human HS tissue and HS-like tissue.

RESULTS:

The structure of PLA/PGA scaffolds facilitates HSFBs adhesion and proliferation. The HSFBs-PLA/PGA composites were in vivo cultured for 12 weeks, and then HS-like tissues were harvested from nude athymic mice. There was no statistical significance in the expression of type I collagen, cell cycle, and cell proliferation between human HS tissue and HS-like tissue.

CONCLUSION:

The authors successfully established a model of human HS using the tissue engineering method, which could provide HS-like tissue for research. And it also could provide enough HS-like tissues to help reduce experimental variability within groups. This model can be used to investigate in prevention and treatment of HS and further explore the mechanisms of HS.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cicatriz Hipertrófica Límite: Animals / Humans Idioma: En Revista: J Craniofac Surg Asunto de la revista: ODONTOLOGIA Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cicatriz Hipertrófica Límite: Animals / Humans Idioma: En Revista: J Craniofac Surg Asunto de la revista: ODONTOLOGIA Año: 2024 Tipo del documento: Article