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Novel Escherichia coli-Infecting Bacteriophages Isolated from Uganda That Target Human Clinical Isolates.
Nale, Janet Yakubu; Chan, Benjamin; Nnadi, Nnaemeka Emmanuel; Cheng, Jeffrey Kwok Jone; Matts, Susan; Nezam-Abadi, Neda; Turkington, Christopher Jason Richard; Charreton, Lucie Manon; Bola, Harroop; Nazir, Ramez; Hoza, Abubakar Shaaban; Wamala, Samuel Posian; Ibanda, Ivan; Maina, Alice Nyambura; Apopo, Auleria Ajiambo; Msoffe, Venance Theophil; Moremi, Nyambura; Moore, Grace Wanjiru; Asiimwe, Ismail; Namatovu, Alice; Mutumba, Paul; Kamya, Deus; Nabunje, Ritah; Nakabugo, Immaculate; Kazwala, Rudovick Ruben; Kangethe, Erastus; Negash, Abel Abera; Watelo, Arthur Kalyebi; Bukamba, Nelson; Muhindo, Gideon; Lubowa, Nathan Musisi; Jillani, Ngalla; Nyachieo, Atunga; Nasinyama, George; Nakavuma, Jesca; Millard, Andrew; Nagel, Tobi Elaine; Clokie, Martha Rebecca Jane.
Afiliación
  • Nale JY; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Chan B; Centre for Epidemiology and Planetary Health, School of Veterinary Medicine, Scotland's Rural College, Inverness, Scotland, United Kingdom.
  • Nnadi NE; Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, USA.
  • Cheng JKJ; Department of Microbiology, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos, Nigeria.
  • Matts S; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Nezam-Abadi N; School of Life Sciences, The University of Warwick, Coventry, United Kingdom.
  • Turkington CJR; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Charreton LM; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Bola H; APC Microbiome Ireland and School of Microbiology, University College Cork, Cork, Ireland.
  • Nazir R; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Hoza AS; APC Microbiome Ireland and School of Microbiology, University College Cork, Cork, Ireland.
  • Wamala SP; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Ibanda I; Sciences et Humanités, École Supérieur de Biologie, Biochimie, Lyon, France.
  • Maina AN; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Apopo AA; Imperial College School of Medicine, London, United Kingdom.
  • Msoffe VT; Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
  • Moremi N; Yale-Waterbury Internal Medicine, Waterbury, Connecticut, USA.
  • Moore GW; Department of Veterinary Microbiology, Parasitology & Biotechnology, College of Veterinary Medicine & Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania.
  • Asiimwe I; Department of Biotechnical and Diagnostic Science, College of Veterinary Medicine, Makerere University, Kampala, Uganda.
  • Namatovu A; Department of Pharmacology and Therapeutics, School of Medicine and Surgery, King Ceasor University, Kampala, Uganda.
  • Mutumba P; Department of Biology, University of Nairobi, Nairobi, Kenya.
  • Kamya D; Department of Food Science and Technology, Technical University of Kenya, Nairobi, Kenya.
  • Nabunje R; Fish Quality Control Laboratory, Kenya Fisheries Service, Nairobi, Kenya.
  • Nakabugo I; Department of Biological Sciences, Mkwawa University College of Education, The Constituent College of University of Dar es Salaam, Iringa, Tanzania.
  • Kazwala RR; Department of Research, National Public Health Laboratory, Dar es Salaam, Tanzania.
  • Kangethe E; Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi, Kenya.
  • Negash AA; Department of Biomolecular Resources and Biolab Sciences, School of Biosecurity, Biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
  • Watelo AK; Department of Biomolecular Resources and Biolab Sciences, School of Biosecurity, Biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
  • Bukamba N; Department of Biomolecular Resources and Biolab Sciences, School of Biosecurity, Biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
  • Muhindo G; Department of Biomolecular Resources and Biolab Sciences, School of Biosecurity, Biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
  • Lubowa NM; Department of Biomolecular Resources and Biolab Sciences, School of Biosecurity, Biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
  • Jillani N; Department of Biomolecular Resources and Biolab Sciences, School of Biosecurity, Biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Kampala, Uganda.
  • Nyachieo A; Department of Veterinary Medicine & Public Health, College of Veterinary Medicine & Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania.
  • Nasinyama G; Department of Public Health, Pharmacology and Toxicology, University of Nairobi, Nairobi, Kenya.
  • Nakavuma J; Armauer Hansen Research Institute, Addis Ababa, Ethiopia.
  • Millard A; Department of Microbiology, Immunology and Parasitology, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia.
  • Nagel TE; Department of Immunology, Uganda Virus Research Institute, Uganda.
  • Clokie MRJ; Mountain Gorilla Veterinary Project Inc, Department of Wildlife and Aquatic Resources Management, College of Veterinary Medicine, Makerere University Kampala, Uganda.
Phage (New Rochelle) ; 4(3): 141-149, 2023 Sep 01.
Article en En | MEDLINE | ID: mdl-37841386
ABSTRACT

Background:

The antimicrobial resistance catastrophe is a growing global health threat and predicted to be worse in developing countries. Phages for Global Health (PGH) is training scientists in these regions to isolate relevant therapeutic phages for pathogenic bacteria within their locality, and thus contributing to making phage technology universally available. Materials and

Methods:

During the inaugural PGH workshop in East Africa, samples from Ugandan municipal sewage facilities were collected and two novel Escherichia coli lytic phages were isolated and characterized.

Results:

The phages, UP19 (capsid diameter ∼100 nm, contractile tail ∼120/20 nm) and UP30 (capsid diameter ∼70 nm, noncontractile tail of ∼170/20 nm), lysed ∼82% and ∼36% of the 11 clinical isolates examined, respectively. The genomes of UP19 (171.402 kb, 282 CDS) and UP30 (49.834 kb, 75 CDS) closely match the genera Dhakavirus and Tunavirus, respectively.

Conclusion:

The phages isolated have therapeutic potential for further development against E. coli infections.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Phage (New Rochelle) Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Phage (New Rochelle) Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido