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Respiratory exposure to agricultural dust extract promotes increased intestinal Tnfα expression, gut barrier dysfunction, and endotoxemia in mice.
Crawford, Meli'sa S; Ulu, Arzu; Ramirez, Briana M; Santos, Alina N; Chatterjee, Pritha; Canale, Vinicius; Manz, Salomon; Lei, Hillmin; Nordgren, Tara M; McCole, Declan F.
Afiliación
  • Crawford MS; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Ulu A; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Ramirez BM; Department of Biochemistry and Molecular Biology, University of California, Riverside, California, United States.
  • Santos AN; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Chatterjee P; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Canale V; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Manz S; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Lei H; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • Nordgren TM; School of Medicine, Division of Biomedical Sciences, University of California, Riverside, California, United States.
  • McCole DF; Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, Colorado, United States.
Am J Physiol Gastrointest Liver Physiol ; 326(1): G3-G15, 2024 01 01.
Article en En | MEDLINE | ID: mdl-37874654
ABSTRACT
Concentrated animal feeding operations (CAFOs) are responsible for the production of global greenhouse gases and harmful environmental pollutants including hydrogen sulfide, ammonia, and particulate matter. Swine farmers are frequently exposed to organic dust that is proinflammatory in the lung and are thus at greater risk of developing pneumonia, asthma, and other respiratory conditions. In addition to respiratory disease, air pollutants are directly associated with altered gastrointestinal (GI) physiology and the development of GI diseases, thereby highlighting the gut-lung axis in disease progression. Instillation of hog dust extract (HDE) for 3 wk has been reported to promote the development of chronic airway inflammation in mice, however, the impact of HDE exposure on intestinal homeostasis is poorly understood. We report that 3-wk intranasal exposure of HDE is associated with increased intestinal macromolecule permeability and elevated serum endotoxin concentrations in C57BL/6J mice. In vivo studies also indicated mislocalization of the epithelial cell adhesion protein, E-cadherin, in the colon as well as an increase in the proinflammatory cytokine, Tnfα, in the proximal colon. Moreover, mRNA expression of the Paneth cell-associated marker, Lyz1, was increased the proximal colon, whereas the expression of the goblet cell marker, Muc2, was unchanged in the epithelial cells of the ileum, cecum, and distal colon. These results demonstrate that airway exposure to CAFOs dusts promote airway inflammation and modify the gastrointestinal tract to increase intestinal permeability, induce systemic endotoxemia, and promote intestinal inflammation. Therefore, this study identifies complex physiological consequences of chronic exposure to organic dusts derived from CAFOs on the gut-lung axis.NEW & NOTEWORTHY Agricultural workers have a higher prevalence of occupational respiratory symptoms and are at greater risk of developing respiratory diseases. However, gastrointestinal complications have also been reported, yet the intestinal pathophysiology is understudied. This work is novel because it emphasizes the role of an inhaled environmental pollutant on the development of intestinal pathophysiological outcomes. This work will provide foundation for other studies evaluating how agricultural dusts disrupts host physiology and promotes debilitating gastrointestinal and systemic disorders.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Endotoxemia / Polvo Límite: Animals Idioma: En Revista: Am J Physiol Gastrointest Liver Physiol / Am. j. physiol. gasterointest. liver physiol / American journal of physiology. Gastrointestinal and liver physiology (Online) Asunto de la revista: FISIOLOGIA / GASTROENTEROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Endotoxemia / Polvo Límite: Animals Idioma: En Revista: Am J Physiol Gastrointest Liver Physiol / Am. j. physiol. gasterointest. liver physiol / American journal of physiology. Gastrointestinal and liver physiology (Online) Asunto de la revista: FISIOLOGIA / GASTROENTEROLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos