Time-lapse live-cell imaging of pyroptosis by confocal microscopy.
STAR Protoc
; 4(4): 102708, 2023 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-37948183
ABSTRACT
Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic expression of gasdermin, cell staining with nuclear and membrane probes, and visualization of pyroptosis by time-lapse imaging. This protocol is applicable to monitoring pyroptosis in various situations. For complete details on the use and execution of this protocol, please refer to Qin et al. (2023).1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Piroptosis
/
Gasderminas
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article