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A sensitive and simple RT-LAMP assay for sarbecovirus screening in bats.
Chan, Tony Tat-Yin; Chow, Franklin Wang-Ngai; Fung, Joshua; Cheng, Flora Ka-Kei; Lo, George Chi-Shing; Tsang, Chi-Ching; Luk, Hayes Kam-Hei; Wong, Antonio Cheuk-Pui; He, Zirong; Aw-Yong, Kam Leng; Liu, Xueyan; Yuen, Kwok-Yung; Woo, Patrick Chiu-Yat; Lau, Susanna Kar-Pui.
Afiliación
  • Chan TT-Y; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Chow FW-N; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Fung J; Department of Health Technology and Informatics, The Hong Kong Polytechnic University , Hong Kong, China.
  • Cheng FK-K; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Lo GC-S; Department of Health Technology and Informatics, The Hong Kong Polytechnic University , Hong Kong, China.
  • Tsang C-C; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Luk HK-H; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Wong AC-P; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • He Z; School of Medical and Health Sciences, Tung Wah College , Hong Kong, China.
  • Aw-Yong KL; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Liu X; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Yuen K-Y; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Woo PC-Y; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
  • Lau SK-P; Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Hong Kong, China.
Microbiol Spectr ; 11(6): e0259123, 2023 Dec 12.
Article en En | MEDLINE | ID: mdl-37971222
ABSTRACT
IMPORTANCE We report the application of a colorimetric and fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay to facilitate mass screening for sarbecoviruses in bats. The assay was evaluated using a total of 838 oral and alimentary samples from bats and demonstrated comparable sensitivity and specificity to quantitative reverse transcription PCR (qRT-PCR), with a simple setup. The addition of SYTO9, a fluorescent nucleic acid stain, also allows for quantitative analysis. The scalability and simplicity of the assay are believed to contribute to improving preparedness for detecting emerging coronaviruses by applying it to field studies and surveillance.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Quirópteros / Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo Límite: Animals Idioma: En Revista: Microbiol Spectr Año: 2023 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Quirópteros / Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo Límite: Animals Idioma: En Revista: Microbiol Spectr Año: 2023 Tipo del documento: Article País de afiliación: China