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AePUb promoter length modulates gene expression in Aedes aegypti.
Anderson, Michelle A E; Leftwich, Philip T; Wilson, Ray; Paladino, Leonela Z Carabajal; Basu, Sanjay; Rooney, Sara; Adelman, Zach N; Alphey, Luke.
Afiliación
  • Anderson MAE; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK. michelle.anderson@york.ac.uk.
  • Leftwich PT; Department of Biology, University of York, Heslington, YO10 5DD, UK. michelle.anderson@york.ac.uk.
  • Wilson R; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.
  • Paladino LZC; School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK.
  • Basu S; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.
  • Rooney S; Department of Biology, University of York, Heslington, YO10 5DD, UK.
  • Adelman ZN; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.
  • Alphey L; Arthropod Genetics, The Pirbright Institute, Ash Road, Pirbright, GU24 0HN, UK.
Sci Rep ; 13(1): 20352, 2023 11 21.
Article en En | MEDLINE | ID: mdl-37990055
ABSTRACT
Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Aedes Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Aedes Límite: Animals Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido