Rapid and robust bacterial species identification using hyperspectral microscopy and gram staining techniques.
J Biophotonics
; 17(4): e202300449, 2024 Apr.
Article
en En
| MEDLINE
| ID: mdl-38176397
ABSTRACT
Gram staining can classify bacterial species into two large groups based on cell wall differences. Our study revealed that within the same gram group (gram-positive or gram-negative), subtle cell wall variations can alter staining outcomes, with the peptidoglycan layer and lipid content significantly influencing this effect. Thus, bacteria within the same group can also be differentiated by their spectra. Using hyperspectral microscopy, we identified six species of intestinal bacteria with 98.1% accuracy. Our study also demonstrated that selecting the right spectral band and background calibration can enhance the model's robustness and facilitate precise identification of varying sample batches. This method is suitable for analyzing bacterial community pathologies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bacterias
/
Microscopía
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Biophotonics
Asunto de la revista:
BIOFISICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China