Your browser doesn't support javascript.
loading
The expression and significance of PD-L1 in condyloma acuminatum.
Liu, Yamei; Huang, Weiqi; Yang, Kun; Du, Xiangxi; Guo, Xueyun; Cao, Yuchun.
Afiliación
  • Liu Y; Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • Huang W; Department of Respiratory and Critical Care Medicine, Union Jiangbei Hospital, Huazhong University of Science and Technology, Wuhan, China.
  • Yang K; Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • Du X; Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • Guo X; Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
  • Cao Y; Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Skin Res Technol ; 30(1): e13558, 2024 Jan.
Article en En | MEDLINE | ID: mdl-38186053
ABSTRACT

BACKGROUND:

It has been reported that programmed death-ligand 1 (PD-L1) is highly expressed in cells during viral infection, which helps the virus escape host immunity. However, the relationship between human papillomavirus (HPV) and PD-L1 in condyloma acuminatum and whether they participate in immunosuppression have not been reported. In this paper, we aimed to explore the expression and significance of PD-L1 in condyloma acuminatum.

METHODS:

The expression of PD-L1 in the wart of condyloma acuminatum patients and the foreskin of healthy individuals was evaluated. Lentivirus transfection was used to introduce the HPV11-E7 gene into HaCaT cells to investigate whether HPV infection could affect the expression of PD-L1. The successfully constructed HPV11-E7 HaCaT cells were cocultured with Jurkat cells, and Jurkat cell apoptosis and proliferation as well as the Jurkat cell cycle were evaluated by flow cytometry and cell counting kit-8 (CCK-8) assays.

RESULTS:

PD-L1 was highly expressed in keratinocytes of genital warts. Through the construction of a cell model, we found that HPV11-E7 could upregulate the expression of PD-L1 in HaCaT cells. Furthermore, HPV11-E7 HaCaT cells can promote the apoptosis of Jurkat cells, inhibit the proliferation of Jurkat cells and mediate the cell cycle arrest of Jurkat cells through the PD-1/PD-L1 signalling pathway.

CONCLUSIONS:

HPV infection may upregulate PD-L1 expression in the keratinocytes of genital warts and participate in the inhibition of local T-cell function.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Verrugas / Condiloma Acuminado / Infecciones por Papillomavirus Límite: Humans Idioma: En Revista: Skin Res Technol Asunto de la revista: DERMATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Verrugas / Condiloma Acuminado / Infecciones por Papillomavirus Límite: Humans Idioma: En Revista: Skin Res Technol Asunto de la revista: DERMATOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China