Dynamic Molecular Markers of Otosclerosis in the Human Cochlea.
Ann Otol Rhinol Laryngol
; 133(4): 390-399, 2024 Apr.
Article
en En
| MEDLINE
| ID: mdl-38197255
ABSTRACT
OBJECTIVE:
To investigate the role and distribution of various molecular markers using immunohistochemistry and immunofluorescence to further elucidate and understand the pathogenesis of otosclerosis.METHODS:
Archival celloidin formalin-fixed 20-micron thick histologic sections from 7 patients diagnosed with otosclerosis were studied and compared to controls. Sections in the mid-modiolar region were immunoreacted with rabbit polyclonal antibodies against nidogen-1, ß2-laminin, collagen-IX, BSP, and monoclonal antibodies against TGF ß-1 and ubiquitin. Digital images were acquired using a high-resolution light and laser confocal microscope.RESULTS:
Nidogen-1, BSP, and collagen-IX were expressed in the otospongiotic regions, and to lesser extent, in the otosclerotic regions, the latter previously believed to be inactive. ß2-laminin and ubiquitin were uniformly expressed in both otospongiotic and otosclerotic regions. There was a basal level of expression of all of these markers in the normal hearing and sensorineural hearing loss specimens utilized as control. TGF ß -1, however, though present in the otosclerosis bones, was absent in the normal hearing and sensorineural hearing loss controls.CONCLUSIONS:
Our results propose that the activity and function of TGF-1 may play a key role in the development and pathogenesis of otosclerosis. Further studies utilizing a higher number of temporal bone specimens will be helpful for future analysis and to help decipher its role as a potential target in therapeutic interventions.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Otosclerosis
/
Pérdida Auditiva Sensorineural
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Ann Otol Rhinol Laryngol
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos