Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish.
STAR Protoc
; 5(1): 102810, 2024 Mar 15.
Article
en En
| MEDLINE
| ID: mdl-38261517
ABSTRACT
The whole kidney marrow (WKM) is the site for hematopoiesis in the adult zebrafish. Here, we present a protocol for analyzing hematopoietic lineages in the WKM of adult zebrafish. We describe steps for the isolation of hematopoietic cells from the WKM, the downstream analysis of total marrow cellularity, and analysis of cell populations by flow cytometry. We then detail procedures for May-Grünwald-Giemsa staining for analysis of cellular morphology and phenotyping. For complete details on the use and execution of this protocol, please refer to Mahony et al.1.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Médula Ósea
/
Pez Cebra
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article