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Lack of Dosage Balance and Incomplete Dosage Compensation in the ZZ/ZW Gila Monster (Heloderma suspectum) Revealed by De Novo Genome Assembly.
Webster, Timothy H; Vannan, Annika; Pinto, Brendan J; Denbrock, Grant; Morales, Matheo; Dolby, Greer A; Fiddes, Ian T; DeNardo, Dale F; Wilson, Melissa A.
Afiliación
  • Webster TH; Department of Anthropology, University of Utah, Salt Lake City, UT, USA.
  • Vannan A; School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • Pinto BJ; School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • Denbrock G; School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • Morales M; Center for Evolution and Medicine, Arizona State University, Tempe, AZ, USA.
  • Dolby GA; Department of Zoology, Milwaukee Public Museum, Milwaukee, WI, USA.
  • Fiddes IT; School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • DeNardo DF; School of Life Sciences, Arizona State University, Tempe, AZ, USA.
  • Wilson MA; Department of Genetics, Yale University, New Haven, CT, USA.
Genome Biol Evol ; 16(3)2024 03 02.
Article en En | MEDLINE | ID: mdl-38319079
ABSTRACT
Reptiles exhibit a variety of modes of sex determination, including both temperature-dependent and genetic mechanisms. Among those species with genetic sex determination, sex chromosomes of varying heterogamety (XX/XY and ZZ/ZW) have been observed with different degrees of differentiation. Karyotype studies have demonstrated that Gila monsters (Heloderma suspectum) have ZZ/ZW sex determination and this system is likely homologous to the ZZ/ZW system in the Komodo dragon (Varanus komodoensis), but little else is known about their sex chromosomes. Here, we report the assembly and analysis of the Gila monster genome. We generated a de novo draft genome assembly for a male using 10X Genomics technology. We further generated and analyzed short-read whole genome sequencing and whole transcriptome sequencing data for three males and three females. By comparing female and male genomic data, we identified four putative Z chromosome scaffolds. These putative Z chromosome scaffolds are homologous to Z-linked scaffolds identified in the Komodo dragon. Further, by analyzing RNAseq data, we observed evidence of incomplete dosage compensation between the Gila monster Z chromosome and autosomes and a lack of balance in Z-linked expression between the sexes. In particular, we observe lower expression of the Z in females (ZW) than males (ZZ) on a global basis, though we find evidence suggesting local gene-by-gene compensation. This pattern has been observed in most other ZZ/ZW systems studied to date and may represent a general pattern for female heterogamety in vertebrates.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Heloderma suspectum / Lagartos / Animales Ponzoñosos Límite: Animals Idioma: En Revista: Genome Biol Evol Asunto de la revista: BIOLOGIA / BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Heloderma suspectum / Lagartos / Animales Ponzoñosos Límite: Animals Idioma: En Revista: Genome Biol Evol Asunto de la revista: BIOLOGIA / BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos