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Generation of a ISL1 homozygous knockout stem cell line (WAe009-A-1G) by episomal vector-based CRISPR/Cas9 system.
Hou, Xiaojie; Fan, Wei; Zeng, Jun; Gao, Zhen; Wan, Juyi; Liao, Bin.
Afiliación
  • Hou X; Department of Cardiovascular Surgery and Cardiovascular Surgery Research Laboratory, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
  • Fan W; Department of Cardiovascular Surgery, The Affiliated Hospital, Southwest Medical University, Metabolic Vascular Diseases Key Laboratory of Sichuan Province, Key Laboratory of Cardiovascular Remodeling and Dysfunction, Luzhou 646000, PR China; Key Laboratory of Medical Electrophysiology, Ministry of
  • Zeng J; Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, (Collaborative Innovation Center for Prevention of Cardiovascular Diseases), Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, Si
  • Gao Z; Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100069, PR China.
  • Wan J; Department of Cardiovascular Surgery, The Affiliated Hospital, Southwest Medical University, Metabolic Vascular Diseases Key Laboratory of Sichuan Province, Key Laboratory of Cardiovascular Remodeling and Dysfunction, Luzhou 646000, PR China; Key Laboratory of Medical Electrophysiology, Ministry of
  • Liao B; Department of Cardiovascular Surgery, The Affiliated Hospital, Southwest Medical University, Metabolic Vascular Diseases Key Laboratory of Sichuan Province, Key Laboratory of Cardiovascular Remodeling and Dysfunction, Luzhou 646000, PR China; Key Laboratory of Medical Electrophysiology, Ministry of
Stem Cell Res ; 76: 103376, 2024 Apr.
Article en En | MEDLINE | ID: mdl-38452706
ABSTRACT
The ISL LIM homeobox 1 (ISL1) gene belongs to the LIM/homeodomain transcription factor family and plays a pivotal role in conveying multipotent and proliferative properties of cardiac precursor cells. Mutations in ISL1 are linked to congenital heart disease. To further explore ISL1's role in the human heart, we have created a homozygous ISL1 knockout (ISL1-KO) human embryonic stem cell line using the CRISPR/Cas9 system. Notably, this ISL1-KO cell line retains normal morphology, pluripotency, and karyotype. This resource serves as a valuable tool for investigating ISL1's function in cardiomyocyte differentiation.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas CRISPR-Cas / Células Madre Embrionarias Humanas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2024 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Sistemas CRISPR-Cas / Células Madre Embrionarias Humanas Límite: Humans Idioma: En Revista: Stem Cell Res Año: 2024 Tipo del documento: Article País de afiliación: China