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ROP GTPases with a geranylgeranylation motif modulate alkaloid biosynthesis in Catharanthus roseus.
Pedenla Bomzan, Dikki; Sharma, Anuj; Lemos Cruz, Pamela; Carqueijeiro, Ines; Bellenger, Léo; Rai, Avanish; Thippesh, Akshay Kumar; Chinnegowda, Venkatesha S; Parihar, Durgesh; Ducos, Eric; Courdavault, Vincent; Nagegowda, Dinesh A.
Afiliación
  • Pedenla Bomzan D; Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru 560065, India.
  • Sharma A; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Lemos Cruz P; Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru 560065, India.
  • Carqueijeiro I; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
  • Bellenger L; Biomolécules et Biotechnologies Végétales, BBV, EA2106, Universitéde de Tours, 37200 Tours, France.
  • Rai A; Biomolécules et Biotechnologies Végétales, BBV, EA2106, Universitéde de Tours, 37200 Tours, France.
  • Thippesh AK; Biomolécules et Biotechnologies Végétales, BBV, EA2106, Universitéde de Tours, 37200 Tours, France.
  • Chinnegowda VS; Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru 560065, India.
  • Parihar D; Department of Biotechnology and Crop Improvement, College of Horticulture, UHS Bagalkot, Mysuru 571130, India.
  • Ducos E; Department of Biotechnology and Crop Improvement, College of Horticulture, UHS Bagalkot, Mysuru 571130, India.
  • Courdavault V; Molecular Plant Biology and Biotechnology Lab, CSIR-Central Institute of Medicinal and Aromatic Plants, Research Centre, Bengaluru 560065, India.
  • Nagegowda DA; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Plant Physiol ; 195(3): 2213-2233, 2024 Jun 28.
Article en En | MEDLINE | ID: mdl-38466200
ABSTRACT
Rho of Plant (ROP) GTPases function as molecular switches that control signaling processes essential for growth, development, and defense. However, their role in specialized metabolism is poorly understood. Previously, we demonstrated that inhibition of protein geranylgeranyl transferase (PGGT-I) negatively impacts the biosynthesis of monoterpene indole alkaloids (MIA) in Madagascar periwinkle (Catharanthus roseus), indicating the involvement of prenylated proteins in signaling. Here, we show through biochemical, molecular, and in planta approaches that specific geranylgeranylated ROPs modulate C. roseus MIA biosynthesis. Among the six C. roseus ROP GTPases (CrROPs), only CrROP3 and CrROP5, having a C-terminal CSIL motif, were specifically prenylated by PGGT-I. Additionally, their transcripts showed higher expression in most parts than other CrROPs. Protein-protein interaction studies revealed that CrROP3 and CrROP5, but not ΔCrROP3, ΔCrROP5, and CrROP2 lacking the CSIL motif, interacted with CrPGGT-I. Further, CrROP3 and CrROP5 exhibited nuclear localization, whereas CrROP2 was localized to the plasma membrane. In planta functional studies revealed that silencing of CrROP3 and CrROP5 negatively affected MIA biosynthesis, while their overexpression upregulated MIA formation. In contrast, silencing and overexpression of CrROP2 had no effect on MIA biosynthesis. Moreover, overexpression of ΔCrROP3 and ΔCrROP5 mutants devoid of sequence coding for the CSIL motif failed to enhance MIA biosynthesis. These results implicate that CrROP3 and CrROP5 have a positive regulatory role on MIA biosynthesis and thus shed light on how geranylgeranylated ROP GTPases mediate the modulation of specialized metabolism in C. roseus.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Regulación de la Expresión Génica de las Plantas / Catharanthus Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Plantas / Regulación de la Expresión Génica de las Plantas / Catharanthus Idioma: En Revista: Plant Physiol Año: 2024 Tipo del documento: Article País de afiliación: India