Kinetics of divalent monoclonal antibody binding to tumour cell surface antigens using flow cytometry: standardization and mathematical analysis.
Mol Immunol
; 22(1): 11-21, 1985 Jan.
Article
en En
| MEDLINE
| ID: mdl-3856096
ABSTRACT
Flow cytofluorimetric methods have been used to quantitate the interaction between a divalent monoclonal antibody and a tumour cell surface antigen. After standardization using fluorescein and 125I-labelled antibodies, kinetics of association and dissociation were measured, and antibody bound at equilibrium quantitated. A mathematical model was developed in conjunction with these experimental results which allowed the calculation of rates for monovalent association and monovalent and divalent dissociation, and a description of the contribution of each to the level of bound antibody at different antibody concns.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Osteosarcoma
/
Anticuerpos Monoclonales
/
Reacciones Antígeno-Anticuerpo
/
Antígenos de Neoplasias
/
Antígenos de Superficie
Límite:
Humans
Idioma:
En
Revista:
Mol Immunol
Año:
1985
Tipo del documento:
Article