Development of an on-site diagnostic LAMP assay for rapid differentiation of the invasive pest Phthorimaea absoluta (Meyrick) using insect tissues.
Pest Manag Sci
; 80(8): 4069-4073, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38563512
ABSTRACT
BACKGROUND:
The tomato leafminer, Phthorimaea absoluta (Meyrick) (Lepidoptera Gelechiidae), is a destructive invasive pest that originated in South America and has spread within China since 2017. A rapid method for on-site identification of P. absoluta is urgently needed for interception of this pest across China.RESULTS:
We developed a loop-mediated isothermal amplification (LAMP) technique to differentiate P. absoluta from Liriomyza sativae, Chromatomyia horticola, and Phthorimaea operculella using extracted genomic DNA, which was then refined to create an on-site LAMP diagnostic method that can be performed under field conditions without the need for laboratory equipment.CONCLUSION:
In the present research, we developed an on-site diagnostic method for rapid differentiation of P. absoluta from other insects with similar morphology or damage characteristics in China. © 2024 Society of Chemical Industry.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Amplificación de Ácido Nucleico
/
Especies Introducidas
/
Mariposas Nocturnas
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Pest Manag Sci
Asunto de la revista:
TOXICOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China