No side effects on rabbit retina or vitreous microenvironment by nd:YAG laser vitreolysis.
BMC Ophthalmol
; 24(1): 171, 2024 Apr 16.
Article
en En
| MEDLINE
| ID: mdl-38627705
ABSTRACT
BACKGROUND:
To explore the safety of NeodymiumYttrium-aluminum-garnet (NdYAG) laser vitreolysis based on the histological examination of the retina and the alteration of vitreous cytokines in the rabbits.METHODS:
Nine male New Zealand rabbits underwent NdYAG laser vitreolysis of 10 mJ x 500 pulses in the left eyes, while the right eyes were used as controls. Intraocular pressure, color fundus photography, and ultrasound B scan were measured before, as well as 1 day, 4 weeks, and 12 weeks after NdYAG laser vitreolysis. Three rabbits were euthanized 1 day, 4 weeks, and 12 weeks after treatment, respectively. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and hematoxylin-eosin (H&E) staining were used to look for pathological changes in the retina. An enzyme-linked immunosorbent assay (ELISA) was utilized to detect the expression of vascular endothelial growth factor (VEGF) and some inflammatory cytokines, including interferon inducible protein 10 (IP-10), monocyte chemoattractant protein 1 (MCP-1) and interlenkin 6 (IL-6) in the vitreous humor. The ascorbic acid (AsA) and total reactive antioxidant potential (TRAP) in the vitreous humor were also measured.RESULTS:
Following NdYAG laser vitreolysis, the levels of VEGF, IP-10, MCP-1, IL6, AsA, and TRAP in the vitreous humor did not change substantially (P > 0.05). There were no detectable pathological changes in the retinal tissues, and no apoptotic signal was found.CONCLUSIONS:
Rabbits tolerate NdYAG laser vitreolysis without observable impact on retinal tissue or the microenvironment of the vitreous.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oftalmopatías
/
Terapia por Láser
/
Láseres de Estado Sólido
Límite:
Animals
Idioma:
En
Revista:
BMC Ophthalmol
Asunto de la revista:
OFTALMOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China