Rapid molecular identification of Rana dybowskii by species-specific primers.
Anal Biochem
; 692: 115548, 2024 Sep.
Article
en En
| MEDLINE
| ID: mdl-38697593
ABSTRACT
Oviductus Ranae is the dried oviduct from Rana dybowskii, a forest frog species with medicinal, tonic, and cosmetic properties. Due to the high price and resource shortage, counterfeit varieties of Oviductus Ranae often appear in the market. However, traditional identification methods cannot accurately differentiate between Oviductus Ranae and its adulterants. In this study, a rapid molecular identification method has been established. The method involves extracting genomic DNA in just 30 s using filter paper purification, species-specific rapid polymerase chain reaction (PCR) amplification, and finally, fluorescence detection of the products. It can accurately identify Oviductus Ranae and its three common adulterants in about 30 min, making the process simple, fast, and highly specific.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ranidae
/
Especificidad de la Especie
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
2024
Tipo del documento:
Article