Accelerated microbial identification "directly" from positive blood cultures using MALDI-TOF MS: Local clinical laboratory challenges.
Diagn Microbiol Infect Dis
; 109(3): 116306, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38735146
ABSTRACT
Rapid identification of microbial pathogens "directly" from positive blood cultures (PBCs) is critical for prompt initiation of empirical antibiotic therapy and clinical outcomes. Towards higher microbial identification rates, we modified a published initial serum separator tubes-based MALDI-TOF-MS protocol, for blood culture specimens received at a non-hospital based standalone diagnostic laboratory, Bangalore, India (a) "Initial" protocol #1 From 28 PBCs, identification= 39% (Gram-negative= 43% Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa; Gram-positive 36% Enterococcus faecalis, Staphylococcus aureus, Staphylococcus haemolyticus); mis-identification= 14%; non-identification= 47%. (b) "Modified" protocol #2 Quality controls (ATCC colonies spiked in negative blood cultures) From 7 analysis, identification= 100% (Escherichia coli, Klebsiella pneumonia, Klebsiella oxytoca, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus); From 7 PBCs, identification= 57%; mis-identification= 14%; non-identification= 29%. Microbial preparations of highest quality and quantity for proteomic analysis and separate spectra matching reference databases for colonies and PBCs are needed for best clinical utility.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
/
Cultivo de Sangre
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
/
Diagn. microbiol. infect. dis
/
Diagnostic microbiology and infectious disease
Año:
2024
Tipo del documento:
Article